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When joint discomfort occurs after starting B12, it is typically coincidental or related to other factors such as excipients in formulations, injection-site effects, pre-existing musculoskeletal conditions, or rare hypersensitivity reactions rather than a direct effect of the vitamin itself

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In some cases, additional tests may be required to assess the underlying cause of the deficiency

The care and use of all animals were approved by the Ethics Committee of Wenzhou Medical University and conformed to the guidelines set forth by the Chinese National Institutes of Health

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