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The dosing of Epitalon was based on a 15-year study done in Russia

RongS.et al.1994Invasiveness and metastasis of NIH 3T3 cells induced by Met-hepatocyte growth factor/scatter factor autocrine stimulation, 84

Research shows that in senescent cells, p53 levels are maintained low through ubiquitination while its pro-apoptotic activity is repressed through nuclear segregation by FOXO4. The peptide's competitive inhibition restores p53 activity by promoting nuclear exclusion, allowing p53 to execute its pro-apoptotic program and eliminate senescent cells through targeted pathway activation

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Kessler RC, Birnbaum HG, Shahly V, Bromet E, Hwang I, McLaughlin KA et al (2010)

Use fresh diluent and maintain strict sterility throughout reconstitution and injection