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One method is to use reclaimed waterthat which is previously used and has gone through advanced treatmentin cooling tower systems

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Then we tried lowering the temperature of the pool by 2 deg, and they went on strike

This is one area where the triple agonist approach clearly outperforms dual agonism

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Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay