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Each PCR reaction was carried out in triplicate, the housekeeping gene beta-actin was employed, and the variations in the abundances of each target genes transcripts were represented as their ratio to the expression of the reference gene of Danio rerio are given in the Supplementary Table S1

Among others changes in the enteric microbiome and an increased intestinal permeability contribute to an overflow of bacterial metabolites into the liver via the portal vein driving the progression of NAFLD

These functions include: increasing energy