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Reactions were incubated for 2 h at 37 C and fluorescence was measured using ex 365 nm, em 450 nm with Varioskan Flash microplate reader

Based on these findings, together with our experimental observation of enhanced STINGTBK1IFN- activation in the MnUA group, we speculate that the superior immunostimulatory effect of MnUA may result from MSU-mediated NF-B priming, which augments STING pathway activation in the presence of Mn.Collectively, these results suggest that MnUA may promote innate immune activation through the coordinated engagement of NF-B and STING signaling pathways

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J Steroid Biochem Mol Biol 152:171179