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showed that low serum vitamin B 12 levels were observed in individuals taking metformin at doses over 2000 mg/day, and a prevalence of mild neuropathy reaching 22.3% in the metformin-users group compared to 15.1% in the non-metformin users group, consistent with the results of previous studies in the literature [49]

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Thereafter, the BPs were treated with 8 mmol/L GSH (Yeasen, G5931120, China) in PBS (pH 7.4) at 37 C for 24 h

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Using a combination of PCR with primers to the ends of the vector and Sal I/Not I restriction enzyme digestion of mini-prep DNA, it was determined that the average insert size of the library was greater the 3.5 Kb

Metabolic signals such as ghrelin, insulin or glucagon modulate insulin sensitivity, glucose tolerance, lipid metabolism, vascular hemostasis, and even immune responses 120,121