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This would be reminiscent of what is occurring in ALS patient spinal cord motor neurons with loss of cuproprotein function and thus overall function that precedes the eventual death of the neurons themselves (Hilton et al., 2024

BKN is then also upregulated (Bossi, 2011)

Agarose gels were all 1.5% w/v agarose stained with ethidium bromide (Fischer) and imaged on BioRad GelDoc imager

We found that 19 metabolites were significantly increased by MTX, whereas 12 were decreased in response to the drug treatment ( Figure 1A and Supplementary Table 4 )