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Dalmasso, L

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The nanocarrier amount was normalized to the protein concentration in the samples measured by BCA protein assay (Thermo Fischer Scientific)

Some clinical, genetic, and functional evidence suggest that the SMAD tumor suppressor pathway may diverted into potent pro-metastatic factor in breast cancer, and signaling through the SMAD pathway can facilitate breast cancer bone metastasis (106)

Approximately 500 mL of whole blood was transferred to a MAP-K2 EDTA Microcontainer (BD, Franklin Lakes, NJ, United States) on ice and centrifuged at 4C at 4388 g in a pre-chilled ultracentrifuge for 15 min