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Briefly, LV tissues were homogenized in HES buffer (10 mM Hepes-NaOH, 0.25M Sucrose, and 1 mM EDTA-2Na) and then centrifuged at 4000 g for 10 minutes at 4C

Interestingly, CBS is upregulated by transcription factors such as nuclear factor erythroid 2-related factor 2 (NRF2) 17 , SP1/3 18 , and NFY 18 and its upregulation confers resistance against ferroptosis in ovarian cancer cells 17

We thank Christopher Koscher for his assistance with protein expression and purification and Florian Grebien for help with establishing the RPE-1 cell lines stably expressing p53 and p53 mutants

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Statistical analysis All data are represented as means standard error of the mean (SEM) and p 0.05 was accepted a statistically significant difference

M.et al (2014a)