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Additionally, electrophysiological experiments have shown the direct effects of B vitamins on pain transmission pathways in the spinal cord and thalamus, which explains the observed reduction or loss of pain sensation in clinical patients

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Immunoblotting Isolation of total, cytosolic and nuclear protein fractions from brain tissue and cells, sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting were conducted as reported previously 34 , 35