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Apiaceae family as a valuable source of biocidal components and their potential uses in agriculture

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10.1158/1078-0432.CCR-12-2334 105 HassaneinM.QianJ.HoeksemaM

Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator