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One significant positive in this study is that they also used laser Doppler scanning (recovery of blood flow in the ischemic muscle) to indicate the promotion of angiogenesis alongside Western blot and real-time PCR for the expression of VEGFR2

The binding of VEGF-A to VEGFR2 is crucial for angiogenesis

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We also quantified metabolites involved in the most proximal stages of the glycolysis pathway, namely glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P) ( Figures 5H, I)

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