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In vitro and in vivo effects of IGF-1 delivery strategies on tendon healing: a review

Figure 8 3.8 qPCR verification To validate the reliability of the transcriptome sequencing data, 16 genes, including seven upregulated and nine downregulated genes, were selected for qRT-PCR analysis

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Another option is to use recombinant Cas9 proteins, which produce mutations at on-target sites as soon as they are introduced into cells and are promptly eliminated by cellular protease machinery, reducing off-target effects [315]