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The isolated immunoprecipitates underwent a series of washes with cell lysis buffer (20 mM Tris, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40) for five cycles, followed by three washes with TBS

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That evening (got the injection at 10am), my arm had intense pain

The exact address will be shared when you schedule your appointment

[1] Anatomic Sites Anatomic sites must be selected carefully for intramuscular injections and include the ventrogluteal, vastus lateralis, and the deltoid