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Regulation of autophagy during ECM detachment is linked to a selective inhibition of mTORC1 by PERK

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AndersenMEButenhoffJLChangSCFarrarDGKennedyGLJrLauCet al

Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight

discoloration, clumping, or moisture inside the vial before reconstitution indicates compromised integrity and the batch should not be used

Overall, these findings suggest that TAB interventions may have significant implications for addressing reproductive dysfunctions associated with elevated MGO/AGEs levels