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About 1L cultured cells were collected and resuspended in 1x PBS with 1% Triton X-100 and 1 mM PMSF, and then lysed with a Microfluidizer Processor (Microfluidics, Model M110L)

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However, both NTI and nor-BNI significantly abolished GHRP-2-induced analgesia, while -FNA had no significant effect