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In brief, cDNA was generated using M-MLV reverse transcriptase (Thermo Fisher) from RNA samples isolated from the cultures, and then incubated with Fast SYBR Green Master Mix (Applied Biosystems)

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Recently, KD feeding was shown to reverse the tactile allodynia produced in a mouse model of metabolic syndrome 64 (strikingly, the KD in this study had almost twice the fat level of the high-fat/moderate carbohydrate diet that induced metabolic syndrome)

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