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Following total RNA extraction, mRNA selection was performed using NEB Next Poly(A) mRNA Magnetic Isolation Module and cDNA Library was prepared with NEB E7760 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs E7490) according to manufacturers protocol

Although results may not be instant, improvements can be observed within a relatively short period

FUS is currently FDA-approved for treating central nervous system diseases, including essential tremor and tremor-related Parkinson's disease

The finding: finasteride responders (patients with clinically visible regrowth) showed increased IGF-1 mRNA in their DPCs

Walking is encouraged during a prolonged fast, but intense exercise such as weight training is best avoided

Wolff et al., 1983)