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Biomonitoring human exposure to environmental carcinogenic chemicals

While another group recently determined a target of the autophagy inhibitor indophagolin using a TPP-based strategy (58), to our knowledge few other studies have used TPP to identify the effector targets of any small molecule inhibitorrather than off-target binders of well-characterized compoundsin human cells

J Eur Acad Dermatol Venereol 36(8):1246-1255, 2022

The day of the experiment, cells were treated with test compounds for the indicated times, then harvested by trypsinization, resuspended in 500 L Hanks Balanced Salt Solution (HBSS, Gibco, Invitrogen Corp., Carlsbad, Calif.) containing either H 2 DCFDA (25 M), C11-BODIPY(581/591) (2 M) or MitoSOX (5 M) (all from Molecular Probes, Invitrogen) and incubated for 10 minutes at 37 C