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g sodium bicarbonate was dissolved in 900 ml water, pH was adjusted to 8.4 with 5 mol/L NaOH pyridine acetonitrile solution per 1 ml acetonitrile contains 25 l of pyridine derivatization reagent solution 0.5% (+) -methyl acetic acid buffer 3 ml glacial acetic acid was dissolved in 900 ml water, pH was adjusted with 5 mol/L NaOH to 7.0, added water to 1000 mL) to the scale, shaken and felted right after remove from the bath

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