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Description

E.MarcosA

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AA: Conceptualization, Data curation, Formal Analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review & editing

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SUMMARY POINTS

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Materials and Methods Cell lines and culture conditions Certified glioma cell lines U87MG and U343MG (p53 wild-type), U251MG (273Arg-His homozygous p53 mutation) and U138MG (273Arg-His heterozygous p53 mutation) were a kind gift from Bernd Kaina (Mainz, Germany), and were routinely grown in DMEM (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Cultilab, Campinas, Brazil) and 1% antibioticantimycotic at 37 C in a humidified, 5% CO 2 atmosphere

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Therefore, businesses must remain vigilant to ensure compliance with various state laws

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Fer-1 did, however, prevent erastin-induced accumulation of cytosolic and lipid ROS ( FIG

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