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Our study revealed that a decline in ATP leads to multiple adverse effects: (1) A shutdown of the ubiquitination cascade occurred and was caused by inhibition of ubiquitin adenylation carried-out in an ATP-dependent manner by the E1 ubiquitin activating enzyme

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Next steps for researchers: review the product COA, run a brief pilot to confirm dosing and assay compatibility, and document storage and handling for every lot to protect data integrity