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Peptides can mimic physiological hormones or binding motifs and often act on a single receptor or pathway, which can reduce off-target effects

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Subsequently, the immunoprecipitates were further washed with kinase buffer containing 20 mM MgCl, 100 M ATP, 2 mM DTT, and 50 M of specified inhibitors, in addition to 40 mM Tris-HCl (pH 7.5)

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