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The samples were collected into procoagulant tubes, allowed to clot for 30 min at room temperature, and then centrifuged at 3000 rpm for 15 min

Images were analysed using FIJI software 48 and the JaCoP plugin 49 was used to calculate Pearsons correlation coefficients of the measured fluorescent signal in the green and red channel

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Perhaps the presence of a branched amino acid at residue 15 constrains SA binding in a particular conformation and functions in organizing the binding pocket

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Forensic Science International , 365 , 112286