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ROS is consisted of free radicals such as superoxide anion radical (O 2 - ) and non-free radical ROS, including hydrogen peroxide (H 2 O 2 ) [50]

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The most concerning aspect is that certain EMS patients are unable to generate qualifying eggs, which reduces the success rate of natural conception and assisted reproduction ( 2 Ovarian reserve and oocyte quality are reduced in EMS patients The essence of EMS is that endometrial cells occur in places other than the uterine cavity, with the most common locations being the surface of tissues or organs such as the ovary, utero-rectal pouch, sacral ligament, bladder, and ureter

The 3D PTC spheroid formation was negatively impacted by the RSL3 treatment (Fig