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Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

T cell activation

In 1850, Commodore John Drake Sloat, in charge of a commission to find a California naval base, recommended the island across the Napa River from the settlement of Vallejo

We also measured the rate of oocyte fragmentation during parthenogenetic activation of aged oocytes in three groups

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