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The homozygosity of T-DNA insertion mutants was verified by PCR using T-DNA specific and target specific primers (Supplementary Table S2)

This regulatory pathway has also been studied and reviewed extensively (6, 17, 18, 29, 63, and references contained therein)

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Iron in infection and immunity

Pharmacological treatment of BD, which is of great importance in the clinical management of BD, has also critical effects on gut microbiota, opening the opportunity to use this knowledge as potential biomarkers