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A troche is placed under the tongue or between the cheek and gum and allowed to dissolve slowly over 15 to 30 minutes

The avidin-biotinylated peroxidase complex (ABC-kit) and DAB substrate (ab64238) were used in accordance with the manufacturers instructions to detect the reaction

[45] Sarangarajan R, Meera S, Rukkumani R, Sankar P, Anuradha G

The core direction of future research should focus on directly verifying the causal relationship between local joint ferroptosis and gut microbiota dysbiosis through germ-free OA mouse models, fecal microbiota transplantation experiments, or conditional knockout models of key genes for chondrocyte-specific ferroptosis (such as GPX4 and ACSL4)

The recommendation to intentionally restrict dietary protein to improve bone health is unwarranted, and potentially even dangerous to those individuals who consume inadequate protein