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Note the different shapes of the concentration-time course (exponentially decreasing) and the effect-time course (linearly decreasing in its central portion)

However, observational studies suggest that whilst acute toxicity remains exceptionally rare, sustained very high levels of B12particularly from supplements or injectionsmay be associated with certain health concerns, though these are correlations rather than proven causal relationships

NAD and the aging process: Role in life, death and everything in between

PMID 20445536.

In reconstituted form, store in fridge and use in 4-8 weeks

Lipid-based vehicles penetrate significantly better than water-based serums