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USA 114 , 1373213737 (2017)

Granulovacuolar degeneration (GVD) bodies of Alzheimer's disease (AD) resemble late-stage autophagic organelles: granulovacuolar degeneration bodies and autophagy

"CuZnSOD deficiency leads to persistent and widespread oxidative damage and hepatocarcinogenesis later in life"

The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)

Briefly, samples were prepared by adding Assay Buffer and processed through heating and centrifugation

10.1016/j.molliq.2022.119597 19 DeepasreeK.VenugopalS