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The resulting protein samples were denatured using 100 l of 2 SDS loading buffer and separated via 12% SDSPAGE

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The central challenge for this fungal pretreatment, however, is economic: the process is often slower and less amenable to continuous industrial operation than established physicochemical methods (e.g., steam explosion, acid hydrolysis), raising concerns about capital costs and volumetric productivity at scale (Zhu et al., 2024)

Additionally, transcriptomic high-throughput sequencing was utilized to acquire the post-treatment inflammatory transcriptomic profile and reveal key regulatory pathways and mechanisms of action