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Cumulative studies have demonstrated that iron chelators and small molecule inhibitors of ferroptosis have protective effects in various AKI animal models [138, 139]

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Small sample sizes: Published studies have involved relatively small numbers of subjects (dozens, not thousands)

To identify potential protein biomarkers associated with a disease status, plasma was collected from two independent Congolese cohorts, a discovery cohort (n = 60) and validation cohort (n = 204), sampled 10 years apart and subjected to multiple high-throughput assays

This is usually accomplished through mechanical crushing techniques