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Theranostics (2020) 10(21):9789807

Rudolf Ruman who made important contributions to this work

Transient overexpression of hs GNMT and associated mutants was achieved with calcium phosphate transfection of the corresponding pcDNA3.3 mammalian overexpression plasmid, which was modified to include an internal ribosome entry site (IRES) downstream of the open reading frame to allow for bicistronic expression of GFP as a fluorescent marker of overexpression

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