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Description

A.ParkY

The simplicity with which GST-fusion proteins can be purified to homogeneity in a one-step affinity chromatography using glutathione immobilized to a matrix (or support) and glutathione itself serving as the eluant further lends support to the choice of GST

10.1007/s00204-022-03365-4 119 VivekS

However, the role of TNF- in the liver is very complex, on the one hand, high doses of TNF- lead to hepatocyte death, on the other hand, it is an essential factor affecting liver regeneration and hepatocyte proliferation

Cells were resuspended in 250 l relevant culture medium supplemented with rho-kinase inhibitor Y-27632 (overnight) and optionally long term with 5 nM scTS2/16