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Furthermore, probiotics help maintain immunological equilibrium by attenuating pathological inflammation through the inhibition of pro-inflammatory cytokine signaling and suppression of the nuclear factor-B (NF-B) pathway (253)

After washing, membrane was incubated with IRDye 800CW Donkey anti-Rabbit Secondary Antibody (1:10,000) or IRDye 680RD Donkey anti-Mouse IgG Secondary Antibody (1:10,000) at room temperature for 1 h