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The antigenantibody complex was bound to protein A/G magnetic beads for 1 h at 25 C, washed with IP Lysis/Wash buffer and purified water, eluted with the antigen/antibody complex, and analyzed using standard immunoblotting protocols

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Activities from freeze-dried extracts were overestimated (Figure 4b) whereas activities from freeze-dried tissue were underestimated (Figure 4c)

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During the implementation of radiotherapy, the dosage of STING agonists should be minimized