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Then, 15 l of the sample was extracted with 25 l of a 50:50 mixture of acetonitrile and methanol containing 12.5 ng ml 1 of caffeine as internal standard for 5 min at 2,000 rpm on an Eppendorf MixMate vortex mixer

& Kawakami, H

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Research has increasingly highlighted the relationship between gut health and the symptoms of ME/CFS, particularly chronic inflammation and dysbiosis, which may exacerbate fatigue and cognitive issues [1, 5, 6]

doi: 10.1007/s13594-015-0211-z 47 YiDYKimSY

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