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Abbreviations ACN: Acetonitrile CE: Collision energy CEES: 2-Chloroethyl ethyl sulfide CID: Collision-induced dissociation Cys: Cysteine DMEM: Dulbecco's Modified Eagle Medium DPBS: Dulbecco's Phosphate Buffered Saline ESI: Electrospray ionization ETETE: Ethylthioethylthioethyl linker FA: Formic acid FRC: Fraction collector GCMS/dFPD: Gas chromatographymass spectrometer/ dual flame photometric detector -GT: -Glutamyltransferase glu-ala-glc: -Glutamyl-alanyl-glycine GSH: Glutathione GST: Glutathione S-transferases HD: Bis(2-chloroethyl sulfide) HETE: Hydroxyethylthioethyl moiety HETETE: Hydroxyethylthioethylthioethyl moiety HPLC: High-performance liquid chromatography LC-HRMS: Liquid chromatography/high resolution mass spectrometry LCMS/MS: Liquid chromatographytandem mass spectrometry LC-Q-TOF/MS: Liquid chromatography quadrapol time-of-flight mass spectrometry Mp: Melting Point MRM: Multiple reaction monitoring mode NAC: N -Acetyl-l-cysteine NAT: N-acetyltransferase PAD: Photodiode array detector PBS: Phosphate Buffered Saline Q: Sesquimustard References Bennett GM, Whincop EM (1921) Some derivatives of monothioethylene glycol

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