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chemical entities e.g., nicotinamide riboside, nicotinamide mononucleotide the invention encompasses a method of providing muscular therapy by co-administering a therapeutically effective amount of one or more NNMT inhibitors with one or more chemical entities (e.g., nicotinamide riboside, nicotinamide mononucleotide) that increase intracellular NAD+ levels to provide muscular therapy

The reaction was initiated by adding 4 L of 25 M stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37 C