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GPR43 activation by SCFAs reduces intracellular ATP depletion and reactive oxygen species (ROS) production, which are critical for NLRP3 assembly (132, 133)

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Levels of mouse cytokines were quantified in the culture supernatants by using IL-6, IFN-, IL-12p40 specific sandwich enzyme-linked immunosorbent assay (ELISA) following the manufacturers instructions (cytokine mouse uncoated ELISA kit, Invitrogen) as described 27

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