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Roshanbin, S

Its reactive metabolites (e.g., methyldiazonium ions, CH 3 N 2 + ) directly interact with DNA bases, resulting in alkylation damage and subsequent gene alterations that promote carcinogenesis.The primary targets of DNA alkylation are the O6 and N7 positions of guanine, as well as the N3 position of adenine.Following MNNG exposure, the primary adduct identified in double-stranded DNA was N7-methylguanine (N7-MeG) at 67%, along with the minor adducts N3-methyladenine (N3-MeA) (12%) and O 6 -methylguanine (O 6 -MeG) (7%) (15)

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In summary, these findings suggest that inhibiting ROS with NAC downregulates HIF-1 and BNIP3/NIX-mediated mitophagy, thereby exacerbating cell apoptosis and inflammation in M9a+sh EPAS1 cells

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