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TAA has shown pathological toxic effects in kidneys, resulting in the deposition of collagen in the renal medulla and fibrin in the tubules 8 , cell death in the terminal portion of the proximal renal tubules 7 , and the renal tissue infiltration of inflammatory cells, degeneration, sclerosis and necrosis of the glomeruli, interstitial fibrosis, and epithelial shedding 11

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After pre-culture, the medium was replaced by 1 ml of fresh culture medium supplemented with the NAs of interest or the respective technical positive and negative reference items