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Maintenance phase (post week 8)

Supernatant containing the protein portion was added with sample buffer (0.125 M Tris [pH 6.8], 10% [v/v] glycerol, 0.02% SDS, and 1 mM dithiothreitol [DTT]) and subsequently heated at 95 C for 5 min

In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans

This is generally done over 5 weeks, although it may need to be renewed once depending on your metabolism

When levels drop below optimal ranges, the body signals distress through fatigue, weakness, and neurological complications

13,14,15 Currently, two main strategies exist to address the application challenges of NADs