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After washing the filters in PBST, TRAF protein binding was analyzed by incubation of the filters with TRAF6 (C-20, #sc-6223, Santa Cruz Biotech., used at 1:1000) or TRAF2 (C-20, #sc-876, Santa Cruz Biotech., used at 1:1000) primary and the respective Horseradish peroxidase-coupled secondary antibodies (Dianova, #305-035-003, used at 1:500, or Cell Signalling Technology, #7074, used at 1:5000)

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In a mouse model, the AC6 C 1 C 2 construct was shown to effectively improve cardiac dysfunction caused by prolonged AR stimulation (Roth et al., 2002)