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The study planned to include 200 patients with type 2 diabetes who were not on any antidiabetic drugs in the 90 days prior to Visit 1 (except in certain clinical situations, such as acute illness, hospitalization, or elective surgery, short-term (14 days) use of insulin)

Antibodies were diluted in a blocking solution

Furthermore, our single-cell mass cytometry approach was limited to ~40 proteins, capping the number of metabolic pathways we could interrogate simultaneously

Both approaches commonly act on key signaling pathways, including Nrf2, NF-B, and AMPK, indicating a potential for synergistic interaction

Conclusions Our findings reveal a growing trend toward digital integration in rare disease trials to support decentralized, scalable, and patient-centered research models

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