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Phosphate buffered saline (without Ca2+ and Mg2+) containing 5% fetal calf serum (FCS) 5 mM EDTA and 5 g/ml DNase I

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For testing efficacy of peptide inhibitor, A 40 was premixed with equal volume of PBS or peptide as indicated, and incubated for 24 h at room temperature with shaking (50 rpm) before being added to the cultures

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alba group and only 18% in the placebo group, with results being statistically and clinically significant ( p 4 g/day) can lead to GI discomfort