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Ethics declarations Conflict of interest The authors declare that they have no competing interests to declare

133 (11 Suppl 2): 3972S3980S

SinhaAMaYScherzerRRahalkarSNeilanBDCraneHet al

which was used as the internal reference gene

The eluted proteins were subjected to size-exclusion chromatography (SEC) with a Superose 6 Increase 10/300 GL column (Cytiva) in the final buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl and 1 mM DTT (buffer A, for biochemical assays) or 5 mM DTT (buffer B, for cryo-EM)