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Labelling of the permeabilized cells with large molecules After permeabilization, cells were incubated with 50 g/mL Texas red-conjugated 70 kDa dextran (lysine fixable, Fisher Scientific, D1864) in growth L15 culture medium for 30 min at 25 C

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Correspondingly, Gdf11 -null mice exhibit anterior homeotic transformations of the vertebrae by displaying an increase in the number of thoracic and lumbar vertebrae and vertebrosternal ribs 9,48