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3a and Supplementary Figures S3ab, S4ad, S5ab)

Global alterations in DNA methylation have revealed a link between the transcriptional dysregulation of Bdnf and its promoter methylation, with Bdnf being a key factor downregulated in HD patients and various HD models

k , Immunoblot of SLC25A39 and ATF4 proteins in HeLa cells treated with indicated doses of mitoCDNB, diamide and BSO (1 mM)/erastin (5 M) for 24 h

for 3 h to reach the mid-exponential phase

The solution was then placed in a 0.1 cm quartz cuvette and scanned using a CD spectrometer (J-815, JASCO Corporation, Japan) with the following parameters: scanning wavelength range of 185260 nm, scanning speed of 100 nm per min, bandwidth of 1.0 nm, response time of 0.50 s, and step resolution of 1.0 nm

Bacteriostatic water serves as the preferred reconstitution solvent for GHK-Cu