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One approach involves labeling the proteins with agents that generate a signal when brought into close proximity by the interaction

Finally, the MDA contents were measured by thiobarbituric acid (TBA) reaction with a Malondialdehyde (MDA) Assay Kit (Solarbio Science & Technology Co., Ltd., China, BC0025) based on the manufacturers instructions

In brief, the samples were first transferred to a 65 C water bath (GFL) for 5 min, before being transferred to 80 C for 5 min

Secondly, many natural products have disadvantages such as poor water solubility, limiting their wider application